Two new genera and species Abropelecinus annulatus gen. et sp. nov. and Azygopelecinus clavatus gen. et sp. nov., placed in the subfamily Iscopininae of the family Pelecinidae, are described and illustrated. Sinopecinus viriosus Zhang, Rasnitsyn & Zhang, 2002 are re-described. All these male specimens were collected from the Yixian Formation of Beipiao City, Liaoning Province, northeastern China. A key to the male species of the subfamily Iscopininae is given. In addition, sexual dimorphism in Pelecinidae and the paleoclimate of the Yixian Formation are briefly discussed.