The study of the Early Miocene (Late Ottnangian/Early Karpatian) ostracod fauna from the Sadovi section (Mt Pozeska gora, Croatia) led to several results concerning Neogene paleobiogeography and paleoecology. Brackish deposits of Late Ottnangian and Early Karpatian age have been recognized for the first time in the North Croatian Basin. These deposits indicate the first marine ingression into the Early Miocene lake in this area. Twenty-nine ostracod species were determined, including the new taxa Fabaeformiscandona slavonica nov. sp. and Herpetocypris sadovii nov. sp.